Uric Acid

The Synermed® uric acid reagents are provided as two ready-to-use liquid stable reagents to enable the use of a dynamic serum blank for minimal interference from serum chromatic substances.

- - - Liquid-Stable Ready-to-Use Reagents
    - Minimal Interference from Serum Bilirubin, Hemoglobin and Lipemia
    - Extended "On-Board" Stability

Principles of the Reaction

The Synermed Uric Acid reagent system determines uric acid concentrations enzymatically using uricase and peroxidase. The hydrogen peroxide formed reacts with a near infrared chromogen that is quantitated at 600 nm.

uricase
Uric Acid + O2 + H2O -> Allantoin + CO2 + H2O2

POD
H2O2 + H3+O + Azure D-2 -> Azure D2 chromophore

Special Performance Characteristics

The Synermed uric acid methodology was correlated to a commercially available enzymatic uric acid method. The calculated linear regression on 49 samples ranging from approximately 2 to 10 mg/dL with Synermed results on the Y-axis was Y = 0.94X + 0.04 with a correlation coefficient of 0.998.
The sensitivity of the procedure is such that an absorbance change of 0.001 will detect as little as 0.14 mg/dl uric acid.
The average within run precision of the method was determined by assaying 35 samples of quality control material at two levels of uric acid concentration:

Mean SD CV

5.0 mg/dl (298 µmol/L) 0.05 (3.0) 1.1%

9.6 mg/dl (571 µmol/L) 0.05 (3.0) 0.5%
The run to run reproducibility of the method as applied to an automated analyzer was determined from the values obtained by 5 replicate analyses of quality control material assayed over a 23 batch period.

Mean SD CV

4.8 mg/dl (286 µmol/L) 0.23 (14) 4.8%

9.8 mg/dl (583 µmol/L) 0.39 (23) 4.0%

Product Packaging and Storage

Cat No.	Product Name	Packaging	Package Volume (mL)	Storage
IR460-X	Uric Acid	1x250 mL + 1x100 mL	350	0-4° C
IR460	Uric Acid	3x250 mL + 3x100 mL	1,050	0-4° C
IR461-L	Uric Acid Chromogen	6x1000 mL	6,000	0-4° C
IR462-L	Uric Acid Enzyme	6x500 mL	3,000	0-4° C
IR460-911	Uric Acid	6x50mL + 3x40mL	420	0-4° C
IR460-WK	Uric Acid	3x25mL + 3x10mL	105	0-4° C

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Principles of the Reaction

Analytical Range

Expected Values

Special Performance Characteristics

SDS

Product Packaging and Storage

Mean	SD	CV
5.0 mg/dl (298 µmol/L)	0.05 (3.0)	1.1%
9.6 mg/dl (571 µmol/L)	0.05 (3.0)	0.5%

Mean	SD	CV
4.8 mg/dl (286 µmol/L)	0.23 (14)	4.8%
9.8 mg/dl (583 µmol/L)	0.39 (23)	4.0%

Uric Acid

Principles of the Reaction

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