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The Synermed® uric acid reagents are provided as two ready-to-use liquid stable reagents to enable the use of a dynamic serum blank for minimal interference from serum chromatic substances.
The Synermed Uric Acid reagent system determines uric acid concentrations enzymatically using uricase and peroxidase. The hydrogen peroxide formed reacts with a near infrared chromogen that is quantitated at 600 nm.
uricase
Uric Acid + O2 + H2O -> Allantoin + CO2 + H2O2
POD
H2O2 + H3+O + Azure D-2 -> Azure D2 chromophore
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