Principles of the Reaction
The Synermed Uric Acid reagent system determines uric acid concentrations enzymatically using uricase and peroxidase. The hydrogen peroxide formed reacts with a near infrared chromogen that is quantitated at 600 nm.
Uric Acid + O2 + H2O -> Allantoin + CO2 + H2O2
H2O2 + H3+O + Azure D-2 -> Azure D2 chromophore
Most applications ofthe procedure have been found to be linear from 0 to 30 mg/dL [1700 µmol/L] uric acid.
The normal range for uric acid in adults is reported to be:
Males 3.5 - 8.0 mg/dL (210 - 475 µmol/L)
Females 2.5 - 6.2 mg/dL (150 - 370 µmol/L)
Special Performance Characteristics
- The Synermed uric acid methodology was correlated to a commercially available enzymatic uric acid method. The calculated linear regression on 49 samples ranging from approximately 2 to 10 mg/dL with Synermed results on the Y-axis was Y = 0.94X + 0.04 with a correlation coefficient of 0.998.
- The sensitivity of the procedure is such that an absorbance change of 0.001 will detect as little as 0.14 mg/dl uric acid.
- The average within run precision of the method was determined by assaying 35 samples of quality control material at two levels of uric acid concentration:
|5.0 mg/dl (298 µmol/L)
|9.6 mg/dl (571 µmol/L)
- The run to run reproducibility of the method as applied to an automated analyzer was determined from the values obtained by 5 replicate analyses of quality control material assayed over a 23 batch period.
|4.8 mg/dl (286 µmol/L)
|9.8 mg/dl (583 µmol/L)
Product Packaging and Storage
||Package Volume (mL)
||1x250 mL + 1x100 mL
||3x250 mL + 3x100 mL
||Uric Acid Chromogen
||Uric Acid Enzyme
||6x50mL + 3x40mL
||3x25mL + 3x10mL