Uric Acid
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The Synermed® uric acid reagents are provided as two ready-to-use liquid stable reagents to enable the use of a dynamic serum blank for minimal interference from serum chromatic substances.
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- Liquid-Stable Ready-to-Use Reagents
- Minimal Interference from Serum Bilirubin, Hemoglobin and Lipemia
- Extended "On-Board" Stability
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Principles of the Reaction
The Synermed Uric Acid reagent system determines uric acid concentrations enzymatically using uricase and peroxidase. The hydrogen peroxide formed reacts with a near infrared chromogen that is quantitated at 600 nm.
uricase
Uric Acid + O2 + H2O -> Allantoin + CO2 + H2O2
POD
H2O2 + H3+O + Azure D-2 -> Azure D2 chromophore
Analytical Range
Most applications ofthe procedure have been found to be linear from 0 to 30 mg/dL [1700 µmol/L] uric acid.
Expected Values
The normal range for uric acid in adults is reported to be:
Males 3.5 - 8.0 mg/dL (210 - 475 µmol/L)
Females 2.5 - 6.2 mg/dL (150 - 370 µmol/L)
Special Performance Characteristics
- The Synermed uric acid methodology was correlated to a commercially available enzymatic uric acid method. The calculated linear regression on 49 samples ranging from approximately 2 to 10 mg/dL with Synermed results on the Y-axis was Y = 0.94X + 0.04 with a correlation coefficient of 0.998.
- The sensitivity of the procedure is such that an absorbance change of 0.001 will detect as little as 0.14 mg/dl uric acid.
- The average within run precision of the method was determined by assaying 35 samples of quality control material at two levels of uric acid concentration:
Mean SD CV 5.0 mg/dl (298 µmol/L) 0.05 (3.0) 1.1% 9.6 mg/dl (571 µmol/L) 0.05 (3.0) 0.5% - The run to run reproducibility of the method as applied to an automated analyzer was determined from the values obtained by 5 replicate analyses of quality control material assayed over a 23 batch period.
Mean SD CV 4.8 mg/dl (286 µmol/L) 0.23 (14) 4.8% 9.8 mg/dl (583 µmol/L) 0.39 (23) 4.0%
Product Packaging and Storage
| Cat No. | Product Name | Packaging | Package Volume (mL) | Storage |
| IR460-X | Uric Acid | 1x250 mL + 1x100 mL | 350 | 0-4° C |
| IR460 | Uric Acid | 3x250 mL + 3x100 mL | 1,050 | 0-4° C |
| IR461-L | Uric Acid Chromogen | 6x1000 mL | 6,000 | 0-4° C |
| IR462-L | Uric Acid Enzyme | 6x500 mL | 3,000 | 0-4° C |
| IR460-911 | Uric Acid | 6x50mL + 3x40mL | 420 | 0-4° C |
| IR460-WK | Uric Acid | 3x25mL + 3x10mL | 105 | 0-4° C |
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