Albumin
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Synermed® albumin method is a modification of the standard BCG method and is optimized for detection at 660 nm in the near-infrared region of the spectrum. Detection at 660 nm eliminates the effects of hemolysis and minimizes interference from lipemia. Precision and linearity are improved by optimizing the reagent to perform at sample/reagent ratios at which automated devices are most precise.
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- Superior Dynamic Range
- Liquid-Stable Ready-to-Use
- Near-Infrared Detection
- Highest Precision
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Principles of the Reaction
Albumin binds with bromcresol green to form a colored complex. The absorbance of the complex is quantitated at 660 nm and is directly proportional to the albumin concentration in the sample.
Bromcresol green + albumin -> albumin-bromcresol
(yellow complex) (blue)
Analytical Range
Most applications of this procedure have been found to be linear to 60 g/L (6 g/dL) albumin.
Limitations of the Procedure
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The Synermed albumin methodology was correlated to a widely accepted bromcresol green method. The calculated linear regression on 42 samples ranging from approximately 15 to 45 g/L (1.5-4.5 g/dl) with Synermed on the Y-axis was: Y = 1.01X + 0.2 g/L (0.02 g/dL) with a correlation coefficient of 0.992
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The sensitivity of the procedure is such that an absorbance change of 0.001 will detect as little as 0.04g/L (0.004 g/dL) of albumin.
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The average within-run precision of the method was determined by assaying 30 samples of quality control material at two levels of albumin concentration which provided the following results:
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Special Performance Characteristics
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The Synermed albumin methodology was correlated to a widely accepted bromcresol green method. The calculated linear regression on 42 samples ranging from approximately 15 to 45 g/L (1.5-4.5 g/dl) with Synermed on the Y-axis was: Y = 1.01X + 0.2 g/L (0.02 g/dL) with a correlation coefficient of 0.992
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The sensitivity of the procedure is such that an absorbance change of 0.001 will detect as little as 0.04g/L (0.004 g/dL) of albumin.
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The average within-run precision of the method was determined by assaying 30 samples of quality control material at two levels of albumin concentration which provided the following results:
Mean SD CV 31 g/L (3.1 g/dL) 0.3 (0.03) 0.8% 46 g/L (4.6 g/dL) 0.3 (0.03) 0.6% -
The run to run reproducibility of the method as applied to an automated analyzer was determined from the values obtained by 5 replicate analyses of quality control material assayed over a 22 batch period. The following results were obtained:
Mean SD CV 31 g/L (3.1 g/dL) 0.4 (0.04) 1.2% 47 g/L (4.7 g/dL) 0.6 (0.06) 1.3%
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Product Packaging and Storage
| Cat No. | Product Name | Packaging | Package Volume (mL) | Storage |
| IR010-X | Albumin Reagent | 2x250 mL | 500 | 20-25° C |
| IR010 | Albumin Reagent | 6x250 mL | 1,500 | 20-25° C |
| IR010-L | Albumin Reagent | 6x1000 mL | 6,000 | 20-25° C |
| IR010-911 | Albumin Reagent | 8 x 50 mL | 400 | 20-25° C |
| IR010-WK | Albumin | 4x55mL | 220 | 20-25° C |
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