Albumin

Synermed® albumin method is a modification of the standard BCG method and is optimized for detection at 660 nm in the near-infrared region of the spectrum. Detection at 660 nm eliminates the effects of hemolysis and minimizes interference from lipemia. Precision and linearity are improved by optimizing the reagent to perform at sample/reagent ratios at which automated devices are most precise.

- - - - Superior Dynamic Range
        
        Liquid-Stable Ready-to-Use
        
        Near-Infrared Detection
        
        Highest Precision

Principles of the Reaction

Albumin binds with bromcresol green to form a colored complex. The absorbance of the complex is quantitated at 660 nm and is directly proportional to the albumin concentration in the sample.

Bromcresol green + albumin -> albumin-bromcresol
(yellow complex) (blue)

Limitations of the Procedure

1. The Synermed albumin methodology was correlated to a widely accepted bromcresol green method. The calculated linear regression on 42 samples ranging from approximately 15 to 45 g/L (1.5-4.5 g/dl) with Synermed on the Y-axis was: Y = 1.01X + 0.2 g/L (0.02 g/dL) with a correlation coefficient of 0.992
2. The sensitivity of the procedure is such that an absorbance change of 0.001 will detect as little as 0.04g/L (0.004 g/dL) of albumin.
3. The average within-run precision of the method was determined by assaying 30 samples of quality control material at two levels of albumin concentration which provided the following results:

Special Performance Characteristics

1. The Synermed albumin methodology was correlated to a widely accepted bromcresol green method. The calculated linear regression on 42 samples ranging from approximately 15 to 45 g/L (1.5-4.5 g/dl) with Synermed on the Y-axis was: Y = 1.01X + 0.2 g/L (0.02 g/dL) with a correlation coefficient of 0.992
2. The sensitivity of the procedure is such that an absorbance change of 0.001 will detect as little as 0.04g/L (0.004 g/dL) of albumin.
3. The average within-run precision of the method was determined by assaying 30 samples of quality control material at two levels of albumin concentration which provided the following results:
  
  Mean SD CV
  
  31 g/L (3.1 g/dL) 0.3 (0.03) 0.8%
  
  46 g/L (4.6 g/dL) 0.3 (0.03) 0.6%
4. The run to run reproducibility of the method as applied to an automated analyzer was determined from the values obtained by 5 replicate analyses of quality control material assayed over a 22 batch period. The following results were obtained:
  
  Mean SD CV
  
  31 g/L (3.1 g/dL) 0.4 (0.04) 1.2%
  
  47 g/L (4.7 g/dL) 0.6 (0.06) 1.3%

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Cat No.	Product Name	Packaging	Package Volume (mL)	Storage
IR010-X	Albumin Reagent	2x250 mL	500	20-25° C
IR010	Albumin Reagent	6x250 mL	1,500	20-25° C
IR010-L	Albumin Reagent	6x1000 mL	6,000	20-25° C
IR010-911	Albumin Reagent	8 x 50 mL	400	20-25° C
IR010-WK	Albumin	4x55mL	220	20-25° C

Principles of the Reaction

Analytical Range

Limitations of the Procedure

Expected Values

Special Performance Characteristics

SDS

Product Packaging and Storage

Mean	SD	CV
31 g/L (3.1 g/dL)	0.3 (0.03)	0.8%
46 g/L (4.6 g/dL)	0.3 (0.03)	0.6%

Mean	SD	CV
31 g/L (3.1 g/dL)	0.4 (0.04)	1.2%
47 g/L (4.7 g/dL)	0.6 (0.06)	1.3%

Albumin

Principles of the Reaction

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