Principles of the Reaction
Albumin binds with bromcresol green to form a colored complex. The absorbance of the complex is quantitated at 660 nm and is directly proportional to the albumin concentration in the sample.
Bromcresol green + albumin -> albumin-bromcresol
(yellow complex) (blue)
Analytical Range
Most applications of this procedure have been found to be linear to 60 g/L (6 g/dL) albumin.
Limitations of the Procedure
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The Synermed albumin methodology was correlated to a widely accepted bromcresol green method. The calculated linear regression on 42 samples ranging from approximately 15 to 45 g/L (1.5-4.5 g/dl) with Synermed on the Y-axis was: Y = 1.01X + 0.2 g/L (0.02 g/dL) with a correlation coefficient of 0.992
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The sensitivity of the procedure is such that an absorbance change of 0.001 will detect as little as 0.04g/L (0.004 g/dL) of albumin.
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The average within-run precision of the method was determined by assaying 30 samples of quality control material at two levels of albumin concentration which provided the following results:
Expected Values
The adult normal range for albumin is reported to be 35-50 g/L (3.5-5.0 g/dL)
Product Packaging and Storage
Cat No. |
Product Name |
Packaging |
Package Volume (mL) |
Storage |
IR010-X |
Albumin Reagent |
2x250 mL |
500 |
20-25° C |
IR010 |
Albumin Reagent |
6x250 mL |
1,500 |
20-25° C |
IR010-L |
Albumin Reagent |
6x1000 mL |
6,000 |
20-25° C |
IR010-911 |
Albumin Reagent |
8 x 50 mL |
400 |
20-25° C |
IR010-WK |
Albumin |
4x55mL |
220 |
20-25° C |