The Synermed® γ-GT procedure utilizes a modification of the method developed by Szasz wherein γ-GT catalyzes the transfer of the g-glutamyl group from the substrate g-glutamyl-3-carboxy-4-nitroanilide to glycylglycine yielding 5-amino-2-nitrobenzoate which absorbs at 405-410 nm.
γ-GT
γ-glutamyl-3-carboxy-4- nitroanilide + glycylglycine -> g-glutamyl-glycylglycine + 5-amino-2-nitrobenzoate
The change in absorbance at 405-410 nm due to the formation of the 5-amino-2-nitrobenzoate is directly proportional to the γ-GT activity in the sample.
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