HDL Cholesterol

- - - - Economical
        
        Liquid-Stable Ready-to-Use Reagent
        
        Compatible with the Chromatically Superior Synermed Cholesterol Reagent
        
        Effects of of Lipemia, Bilirubin, and Hemolysis Minimized
        
        Highly Sensitive

Principles of the Reaction

Serum lipids may be separated by treatment with precipitant comprised of dilute polyethylene glycol at an alkaline pH. The larger, lipid-rich lipoproteins, VLDL and LDL form insoluble complexes more readily than do the smaller, protein-rich HDL. The insoluble complexes can be sedimented by low-speed centrifugation.
serum lipids + PEG precipitant -> HDL fraction + [LDL + VDL fractions]
(supernatant) (precipitate)
The supernatant which contains the HDL fraction is then assayed for cholesterol using the Synermed Total Cholesterol reagent kit.

Special Performance Characteristics

The Synermed cholesterol methodology was correlated to a widely accepted enzymatic cholesterol method. The calculated linear regression on 47 samples ranging from approximately 26-106 mg/dl with Synermed results on the Y-axis was: Y = 0.91X + 0.46 mg/dl with a correlation coefficient of 0.978.
The sensitivity of the procedure is such that an absorbance change of 0.001 will detect as little as 0.4 mg/dl of cholesterol.
The average within run precision of the method was determined by assaying 30 samples of quality control material which provided the following results:

Mean SD CV

24.3 mg/dL 0.8 3.4%

99.9 mg/dL 2.7 2.7%
The run to run reproducibility of the method as applied to an automated analyzer was determined from the values obtained by 5 replicate analyses of quality control material assayed over a 22 batch period. The following results were obtained:

Mean SD CV

24.5 mg/dL 1.0 4.1%

112 mg/dL 4.1 3.6%

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Cat No.	Product Name	Packaging	Package Volume (mL)	Storage
IR055-X	HDL Cholesterol precipitating rgt	2x100 mL	200	0-4° C
IR055	HDL Cholesterol precipitating rgt	1x250 mL	250	0-4° C

Principles of the Reaction

Analytical Range

Special Performance Characteristics

SDS

Product Packaging and Storage

Mean	SD	CV
24.3 mg/dL	0.8	3.4%
99.9 mg/dL	2.7	2.7%

Mean	SD	CV
24.5 mg/dL	1.0	4.1%
112 mg/dL	4.1	3.6%

HDL Cholesterol

Principles of the Reaction

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