HDL Cholesterol
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- Economical
- Liquid-Stable Ready-to-Use Reagent
- Compatible with the Chromatically Superior Synermed Cholesterol Reagent
- Effects of of Lipemia, Bilirubin, and Hemolysis Minimized
- Highly Sensitive
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Principles of the Reaction
Serum lipids may be separated by treatment with precipitant comprised of dilute polyethylene glycol at an alkaline pH. The larger, lipid-rich lipoproteins, VLDL and LDL form insoluble complexes more readily than do the smaller, protein-rich HDL. The insoluble complexes can be sedimented by low-speed centrifugation.
serum lipids + PEG precipitant -> HDL fraction + [LDL + VDL fractions]
(supernatant) (precipitate)
The supernatant which contains the HDL fraction is then assayed for cholesterol using the Synermed Total Cholesterol reagent kit.
Analytical Range
The procedure described is suitable for levels of HDL cholesterol up to 100 mg/dl. Any specimen with an HDL cholesterol value greater than this should be diluted with physiologic saline and reassayed. The value obtained must then be corrected for the dilution factor.
Special Performance Characteristics
- The Synermed cholesterol methodology was correlated to a widely accepted enzymatic cholesterol method. The calculated linear regression on 47 samples ranging from approximately 26-106 mg/dl with Synermed results on the Y-axis was: Y = 0.91X + 0.46 mg/dl with a correlation coefficient of 0.978.
- The sensitivity of the procedure is such that an absorbance change of 0.001 will detect as little as 0.4 mg/dl of cholesterol.
- The average within run precision of the method was determined by assaying 30 samples of quality control material which provided the following results:
Mean SD CV 24.3 mg/dL 0.8 3.4% 99.9 mg/dL 2.7 2.7% - The run to run reproducibility of the method as applied to an automated analyzer was determined from the values obtained by 5 replicate analyses of quality control material assayed over a 22 batch period. The following results were obtained:
Mean SD CV 24.5 mg/dL 1.0 4.1% 112 mg/dL 4.1 3.6%
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