Serum Iron
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The Synermed serum iron method is an improvement over most traditional methods in that the detection is in the region of the spectrum where hemoglobin and lipemia have less absorbance than at the wavelength used in traditional methods. The Synermed serum iron is the only automated serum iron method providing reference quality results.
When Is a Serum Iron Really a Serum Iron? A Follow-up Study on the Status of Iron Measurements in Serum. Clinical Chemistry 42, No. 1, 1996 pp 109-111 Norbert W. Tietz, et al.
Excerpts follow:
The [Synermed] method showed good correlation with the ICSH method for specimens with ferritin values 1200 µg/L, the ICSH method showed a positive bias for most values.”
“The ICSH method is subject to interference by ferritin when present in concentrations >1200 µg/L. This observation casts doubt on the suitability of the ICSH method as a reference method for the measurement of serum iron ....”
“Thus, we renew our call for a new Reference Method for measuring serum iron that meets today’s need for accuracy and precision.”
“the accuracy of the Synermed ... method ... is acceptable for use in the clinical laboratory.”
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- Liquid-Stable Ready-to-Use Reagent
- Minimal Interference from Lipemia
- May Be Used with Serum of Dialysis Patients
- Reference Quality Accuracy
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Principles of the Reaction
Serum iron is transported in the ferric oxidation state while bound to transferrin. In the Synermed Serum Iron reagent system, the ferric ion is dissociated from transferrin at an acid pH in the presence of ascorbic acid which reduces the ferric ion to ferrous ion. The ferrous ion then reacts with the chromogen Ferene® to form a blue chromophore. The absorbance of the blue iron-dye complex is proportional to serum iron concentration and is read spectrophotometrically at 600 nm.
H+ ascorbate
Transferrin_2Fe+3 -> transferrin + 2Fe+3 -> 2Fe+2 + dehydroascorbate
Fe+2 + Ferene -> Fe+2_Ferene complex
(yellow) (blue)
Analytical Range
Most applications of the procedure have been found to be linear to 170 µmol/L (900 µg/dL).
Expected Values
The normal range for serum iron in adults is reported to be:
Males 10-29 µmol/L 50-160 µg/dL
Females 7-27µmol/L 40-150 µg/dL
Special Performance Characteristics
- The Synermed serum iron methodology was correlated to a serum blanked bathophenanthroline method. The calculated linear regression on samples ranging from approximately 2 to 50 µmol/L (11 to 280 µg/dL) with Synermed results on the Y-axis is: Y = 1.01X - 1.22 with a correlation coefficient of 0.995.
- The sensitivity of the procedure is such that an absorbance change of 0.001 will detect as little as 0.54 µmol/L (3 µg/dL) serum iron.
- The average within run precision of the method was determined by assaying 30 samples of quality control material at two levels of iron concentration:
Mean SD CV 17.5 µmol/L ( 97.7 µg/dL) 0.48 (2.7) 2.8% 29.4 µmol/L (164.1 µg/dL)) 0.86 (4.8) 2.9% - The run to run reproducibility of the method as applied to an automated analyzer was determined from the values obtained by 5 replicate analyses of quality control material assayed over a 22 batch period. The following results were obtained:
Mean SD CV 21.5 µmol/L (120.0 µg/dL) 0.70 (3.9) 3.3% 41.3 µmol/L (230.5 µg/dL) 0.85 (4.7) 2.1%
Product Packaging and Storage
| Cat No. | Product Name | Packaging | Package Volume (mL) | Storage |
| IR320-X | Serum Iron | 1x250 mL + 1x80 mL | 330 | 0-4° C |
| IR320 | Serum Iron | 3x250 mL + 3x80 mL | 990 | 0-4° C |
| IR321-L | Serum Iron Buffer 600 nm | 6x1000 mL | 6,000 | 0-4° C |
| IR322-L | Serum Iron Chromogen 600 nm | 6x500 mL | 1,500 | 0-4° C |
| IR320-911 | Serum Iron | 4x50mL + 2x29mL | 258mL | 0-4° C |
| IR320-WK | Serum Iron | 3x25mL + 3x7.2mL | 96 | 0-4° C |
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