α-Amylase

- - - - Liquid-Stable
      - Single Ready-to-Use Reagent
      - Improved Sensitivity
      - Minimal Bilirubin Interference
      - No Maltase Interference - Usable with Veterinary Samples

Principles of the Reaction

The substrate 2-chloro-p-nitrophenyl-D-maltotrioside [CNPG3] is hydrolyzed by α-amylase to release 2-chloro-p-nitrophenol [CNP] which is monitored spectrophotometrically at 405 nm. The hydrolysis products also include 2-chloro-4-nitrophenyl-D-maltoside [CNPG2], maltotriose [G3] and glucose [G]. The rate of formation of the 2-chloro-p-nitrophenol is directly proportional to the concentration of α-amylase activity in the sample.

α-amylase
10 CNPG3 -> 9 CNP + 1 CNPG2 + 9 G3 + 1G

Special Performance Characteristics

The Synermed® Amylase methodology was correlated to a commercially available amylase method. The calculated linear regression on 44 serum samples ranging from approximately 9 to 572 U/L with Synermed results on the Y-axis is: Y = 1.02X + 0.14 U/L with a correlation coefficient of 0.999
The sensitivity of the procedure is such that an absorbance change per minute of 0.001 will detect as little as 6 U/L amylase.
The within run precision of the method as applied to an automated analyzer was determined by assaying 30 samples of quality control materials at two levels of amylase concentration yielding the following results:

Mean SD CV

58 U/L 1.2 2.1%

292 U/L 2.8 1.0%
The run to run reproducibility of the method as applied to an automated analyzer was determined from the values obtained by 5 replicate analyses of quality control material assayed over a 21 batch period. The following results were obtained:

Mean SD CV

44 U/L 1.0 2.2%

295 U/L 5.1 1.7%

Go to top

Cat No.	Product Name	Packaging	Package Volume (mL)	Storage
VI400-X	Amylase	1x50 mL	50	0-4° C
VI400	Amylase	2 x 100 mL	200	0-4° C
VI400-L	Amylase	6x250 mL	1,500	0-4° C
VI400-911	Amylase	8x50 mL	400	0-4° C
VI400-WK	Amylase	3x55mL	165	0-4° C

Principles of the Reaction

Analytical Range

Expected Values

Special Performance Characteristics

SDS

Product Packaging and Storage

Mean	SD	CV
58 U/L	1.2	2.1%
292 U/L	2.8	1.0%

Mean	SD	CV
44 U/L	1.0	2.2%
295 U/L	5.1	1.7%

α-Amylase

Principles of the Reaction

Chemical Reagents Catalog

Connect with us

Certifications