Principles of the Reaction
The substrate 2-chloro-p-nitrophenyl-D-maltotrioside [CNPG3] is hydrolyzed by α-amylase to release 2-chloro-p-nitrophenol [CNP] which is monitored spectrophotometrically at 405 nm. The hydrolysis products also include 2-chloro-4-nitrophenyl-D-maltoside [CNPG2], maltotriose [G3] and glucose [G]. The rate of formation of the 2-chloro-p-nitrophenol is directly proportional to the concentration of α-amylase activity in the sample.
α-amylase
10 CNPG3 -> 9 CNP + 1 CNPG2 + 9 G3 + 1G
Analytical Range
Most applications of the procedure have been found to be linear to at least 1,500 U/L amylase.
Expected Values
A reference range for α-amylase in serum in adults measured at 37° C by a similar kinetic method is reported to be 25-125 U/L.
Product Packaging and Storage
Cat No. |
Product Name |
Packaging |
Package Volume (mL) |
Storage |
VI400-X |
Amylase |
1x50 mL |
50 |
0-4° C |
VI400 |
Amylase |
2 x 100 mL |
200 |
0-4° C |
VI400-L |
Amylase |
6x250 mL |
1,500 |
0-4° C |
VI400-911 |
Amylase |
8x50 mL |
400 |
0-4° C |
VI400-WK |
Amylase |
3x55mL |
165 |
0-4° C |