Creatinine
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Traditional Kinetic Jaffé Traditional Kinetic Jaffé methods are subject to severe interference by both bilirubin and hemolysis because the absorbance of these substances changes progressively with time due to oxidation or "bleaching" during the reaction. Synermed VI360 Kinetic Jaffé The Synermed kinetic creatinine decolorizes both bilirubin and hemoglobin. The background absorbance is lower thereby simplifying the spectrophotometric measurement of absorbance change and eliminating negative interference due to loss of absorbance of chromatic substances.
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- No Interference from Serum Bilirubin
- No Interference from Serum Hemoglobin
- Compares to Expensive Enzymatic Methods
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Principles of the Reaction
Creatinine reacts with picrate ions in an alkaline solution to form a red tautomer of creatinine picrate which can be quantitated spectrophotometrically at 500 - 530 nm.
OH-
Creatinine + picric acid -> Creainine-picrate complex
(red)
The Synermed creatinine reagent system utilizes an alkaline picrate solution with an added compound to decolorize endogenous bilirubin, thus eliminating bilirubin interference in the method. The creatinine reaction is measured kinetically to virtually eliminate interferences from Jaffé positive substances.
Analytical Range
Most applications of this procedure have demonstrated linearity to 24 mg/dL (2122 µmol/L).
Limitations of the Procedure
- Conjugated bilirubin levels up to 30 mg/dl have been tested in the Synermed laboratory and produced no interference in this method.
- Unconjugated bilirubin levels up to 30 mg/dl have been tested in the Synermed laboratory and produced no interference in this method.
- Hemoglobin levels up to 1000 mg/dl have been tested in the Synermed laboratory and produced no interference in this assay.
- Lipemia levels up to 1000 mg/dl (as Intralipid) have been tested in the Synermed laboratories and produced no interference.
Expected Values
The normal range for creatinine in adult human serum is 0.7-1.5 mg/dL (62-133 µmol/L).
Special Performance Characteristics
- The Synermed creatinine methodology was correlated to a commercially available alkaline picrate method. The linear regression on 41 samples ranging from approximately 0.6 to 12.4 mg/dl (53-1096 µmol/L) with Synermed results on the Y-axis gave an equation of: Y = 0.97X - 0.03 with a correlation coefficient of 0.999.
- The sensitivity of the procedure is such that an absorbance change of 0.001 will detect as little as 0.18 mg/dL (15.9 µmol/L) creatinine.
- The average within run precision of the method was determined by assaying 30 samples of quality control materials at two levels of creatinine concentration using the Hitachi 705 analyzer which yielded the following results:
Mean SD CV 2.4 mg/dL (212 µmol/L) 0.04 (3.5) 1.6% 7.7 mg/dL (681 µmol/L) 0.14 (12.4) 1.9% - The run to run reproducibility of the method as applied to the Hitachi 705 analyzer was determined from the values obtained by 5 replicate analyses of quality control material assayed over a 20 batch period. The following results were obtained:
Mean SD CV 2.6 mg/dL (230 µmol/L) 0.12 (10.6) 4.7% 9.0 mg/dL (796 µmol/L) 0.22 (19.4) 2.5%
Product Packaging and Storage
| Cat No. | Product Name | Packaging | Packaging Volume (mL) | Storage |
| VI060-X | Creatinine, single reagent | 2x250 mL | 500 | 20-25° C |
| VI060 | Creatinine, single reagent | 6x250 mL | 1,500 | 20-25° C |
| VI260-X | Creatinine, 2 reagent | 1x250 mL + 1x50 mL | 300 | 20-25° C |
| VI260 | Creatinine, 2 reagent | 5x250 mL + 1x250 mL | 1,500 | 20-25° C |
| VI261-L | Creatinine Buffer | 6x1000 mL | 6,000 | 20-25° C |
| VI262-L | Creatinine Picrate Reagent | 6x1000 mL | 6,000 | 20-25° C |
| VI560-X | Creatinine | 1x250 mL + 1x250 mL | 500 | 20-25° C |
| VI560 | Creatinine | 3x250 mL + 3x250 mL | 1,500 | 20-25° C |
| VI561-L | Creatinine Buffer | 6x500 mL | 3,000 | 20-25° C |
| VI562-L | Creatinine Picrate Reagent | 6x500 mL | 3,000 | 20-25° C |
| VI560-911 | Creatinine | 4x50 mL + 4x50 mL | 400 | 20-25° C |
| VI560-WK | Creatinine | 3x25mL + 3x25mL | 150 | 20-25° C |
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