The Synermed Triglycerides reagent system employs the enzymes lipase, glycerol kinase, glycerophosphate oxidase, and peroxidase and measures the formation of hydrogen peroxide. The reaction proceeds to a blue color which is measured spectrophotometrically at 660 nm, thus virtually eliminating chromatic interferences from hemoglobin, bilirubin and lipemia.
Triglycerides are hydrolyzed by lipase to glycerol and free fatty acids.
triglycerides -> glycerol + fatty acids
The glycerol formed reacts with adenosine-5'-triphosphate [ATP] in the presence of glycerol kinase [GK] to form adenosine-5'-diphosphate [ADP] and glycerol-1-phosphate [G-1-P].
glycerol + ATP -> G-1-P + ADP
Glycerol-1-phosphate is then oxidized by glycerophosphate oxidase [GPO] to Dihydroxyacetone phosphate [DHP] and hydrogen peroxide [H2O2].
G-1-P + O2 -> DHP + H2O2
The hydrogen peroxide reacts with the Synermed chromogen in the presence of peroxidase [POD] to form a blue azo dye which is quantitated between 630 and 660 nm.