Principles of the Reaction
The sample containing both conjugated and unconjugated bilirubin is dissolved in the reagent and both conjugated and unconjugated bilirubin are oxidized in the presence of the electron transfer agent KHC3 to form a stable blue-green infrared light absorbing chromophore cholecyanin.
KHC3 Bilirubin -> cholecyanin (yellow) (blue-green) |
The reaction product is detected at 700 nm in the near-infrared region of the spectrum.
Analytical Range
Most applications of the procedure have been found to be linear to 30 mg/dL (513 µmol/L) bilirubin.
Limitations of the Procedure
- The interference from hemoglobin was tested using the application to the IR200 and no interference outside the imprecision of the test was noted up to levels of 500 mg/dL hemoglobin.
- The interference from lipemia (tested as Intralipid) was determined using the application to the IR200 and no interference outside the imprecision of the test was noted up to levels of 500 mg/dl lipemia. See Chart below.
- The interference from a hemoglobin based blood substitute was tested and no interference outside the imprecision of the test was observed up to levels of 1000 mg/dL. See Chart below.
- Urinary indican was tested at levels of 0.5 mM and 1.0 mM and no interference was observed.
Expected Values
Bilirubin levels will vary with age, sex, and diet. It is strongly recommended that each laboratory establish an expected range for the local population. The normal range for total bilirubin in serum is reported to be 0.1-1.4 mg/dl (1.7-23.9 µmol/L).
Product Packaging and Storage
Cat No. |
Product Name |
Packaging |
Package Volume (mL) |
Storage |
IR450-X |
Bilirubin, Total DS |
1x250 mL + 1x50 mL |
300 |
0-4° C |
IR450 |
Bilirubin, Total DS |
3x250 mL + 3x50 mL |
900 |
0-4° C |
IR451-L |
Bilirubin, Total - DS Reagent |
6x1000 mL |
6,000 |
0-4° C |
IR452-L |
Bilirubin, Total - DS Oxidant |
6x250 mL |
1,500 |
0-4° C |
IR700-X |
Bilirubin, Total 700 nm (Human) |
2x250 mL |
500 |
0-4° C |
IR700 |
Bilirubin, Total |
6x250 mL |
1,500 |
0-4° C |
IR700-L |
Bilirubin, Total 700 nm (Vet) |
6x1000 mL |
6,000 |
0-4° C |
IR700-911 |
Bilirubin, Total |
8x50 mL |
400 |
0-4° C |
IR700-WK |
Bilirubin, Total |
4x55mL |
220 |
0-4° C |