Total Bilirubin 700nm

Synermed® IR700 total bilirubin is not based upon the diazo reaction and is therefore not subject to chemical interference from hemoglobin as are all diazo methods. Urinary indicans which are measured by most automated diazobilirubin methods have no effect in the infrared method. Chromatic interferences are minimized by detection at 700 nm where hemoglobin and lipemia have little absorbance. The method is optimized for performing bilirubin assays on sera which contain hemoglobin along with high levels of bilirubin, such as often occurs with pediatric samples.

- - - - Mono Reagent
        
        Ready-to-Use Liquid Reagent
        
        Minimizes Chromatic Interferences
        
        Eliminates Troublesome Negative Values
        
        Room Temperature Stability
        
        Useful After Infusion With Artificial Blood
        
        Reliable Values on Uremic Patients

Principles of the Reaction

The sample containing both conjugated and unconjugated bilirubin is dissolved in the reagent and both conjugated and unconjugated bilirubin are oxidized in the presence of the electron transfer agent KHC3 to form a stable blue-green infrared light absorbing chromophore cholecyanin.

KHC3
Bilirubin -> cholecyanin
(yellow) (blue-green)

The reaction product is detected at 700 nm in the near-infrared region of the spectrum.

Limitations of the Procedure

The interference from hemoglobin was tested using the application to the IR200 and no interference outside the imprecision of the test was noted up to levels of 500 mg/dL hemoglobin.
The interference from lipemia (tested as Intralipid) was determined using the application to the IR200 and no interference outside the imprecision of the test was noted up to levels of 500 mg/dl lipemia. See Chart below.
The interference from a hemoglobin based blood substitute was tested and no interference outside the imprecision of the test was observed up to levels of 1000 mg/dL. See Chart below.
Urinary indican was tested at levels of 0.5 mM and 1.0 mM and no interference was observed.

Special Performance Characteristics

The Synermed IR700 Total Bilirubin methodology as applied to the IR200 analyzer was correlated to the Synermed IR450 Total Bilirubin method . The calculated linear regression on 40 samples ranging from approximately 0.2 to 25.2 mg/dl with Synermed IR 700 results on the Y-axis was: Y = 1.00X + 0.04 mg/dL with a correlation coefficient of 0.998.
The sensitivity of the procedure is such that an absorbance change of 0.001 (as measured in a 1 cm light path) will detect as little as 0.04 mg/dL bilirubin.
The within run precision of the method as applied to the Synermed IR200 analyzer was determined by assaying samples of quality control materials at two levels of bilirubin concentration:

Mean SD CV

0.9 mg/dL 0.06 6.2%

7.1 mg/dL 0.07 1.0%
The run to run reproducibility of the method as applied to the Synermed IR200 analyzer was determined from the values obtained by 5 replicate analyses of quality control material assayed over a 20 batch period over 5 days. The following results were obtained:

Mean SD CV<

1.0 mg/dL < 0.08 < 8.3%

6.8 mg/dL 0.27 3.9%

Product Packaging and Storage

Cat No.	Product Name	Packaging	Package Volume (mL)	Storage
IR450-X	Bilirubin, Total DS	1x250 mL + 1x50 mL	300	0-4° C
IR450	Bilirubin, Total DS	3x250 mL + 3x50 mL	900	0-4° C
IR451-L	Bilirubin, Total - DS Reagent	6x1000 mL	6,000	0-4° C
IR452-L	Bilirubin, Total - DS Oxidant	6x250 mL	1,500	0-4° C
IR700-X	Bilirubin, Total 700 nm (Human)	2x250 mL	500	0-4° C
IR700	Bilirubin, Total	6x250 mL	1,500	0-4° C
IR700-L	Bilirubin, Total 700 nm (Vet)	6x1000 mL	6,000	0-4° C
IR700-911	Bilirubin, Total	8x50 mL	400	0-4° C
IR700-WK	Bilirubin, Total	4x55mL	220	0-4° C

Go to top

Principles of the Reaction

Analytical Range

Limitations of the Procedure

Expected Values

Special Performance Characteristics

SDS

Product Packaging and Storage

Mean	SD	CV
0.9 mg/dL	0.06	6.2%
7.1 mg/dL	0.07	1.0%

Mean	SD	CV<
1.0 mg/dL	< 0.08	< 8.3%
6.8 mg/dL	0.27	3.9%

Total Bilirubin 700nm

Principles of the Reaction

Chemical Reagents Catalog

Connect with us

Certifications