Traditional Kinetic Jaffé Traditional Kinetic Jaffé methods are subject to severe interference by both bilirubin and hemolysis because the absorbance of these substances changes progressively with time due to oxidation or "bleaching" during the reaction. Synermed VI360 Kinetic Jaffé The Synermed kinetic creatinine decolorizes both bilirubin and hemoglobin. The background absorbance is lower thereby simplifying the spectrophotometric measurement of absorbance change and eliminating negative interference due to loss of absorbance of chromatic substances.
Creatinine reacts with picrate ions in an alkaline solution to form a red tautomer of creatinine picrate which can be quantitated spectrophotometrically at 500 - 530 nm.
Creatinine + picric acid -> Creainine-picrate complex
The Synermed creatinine reagent system utilizes an alkaline picrate solution with an added compound to decolorize endogenous bilirubin, thus eliminating bilirubin interference in the method. The creatinine reaction is measured kinetically to virtually eliminate interferences from Jaffé positive substances.